BD, 554707, BD Pharmingen™ APC Rat Anti-Human IL-10

Alternative Name: Interleukin-10; CSIF; Cytokine synthesis inhibitory factor; TGIF
Reactivity: Human (QC Testing), Viral (Tested in Development)
Isotype: Rat IgG2a, κ
Immunogen: Recombinant Human IL-10
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_398582
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The JES3-19F1 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate IL-10 producing cells within mixed cell populations. The APC-conjugated JES3-19F1 antibody (Cat. No. 554707) is especially suitable for these studies (see image). For optimal immunofluorescent staining and flow cytometric analysis, this anti-cytokine antibody should be titrated (≤ 0.5 µg mAb/million cells). For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocol section or the chapter on intracellular staining in the Immune Function Handbook. A useful control for demonstrating specificity of staining is either of the following: 1) pre-block the conjugated JES3-19F1 antibody with ligand (e.g., recombinant human IL-10; Cat. No. 554611) prior to staining, or 2) pre-block the fixed/permeabilized cells with unlabelled JES3-19F1 antibody (Cat. No. 554705) prior to staining. The staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. A suitable rat IgG2a isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized human cells is PE-R35-95 (Cat. No. 554690); use at comparable concentrations to the antibody of interest (e.g., ≤ 0.5 µg mAb/1 million cells).