BD, 555088, BD Pharmingen™ PE Mouse Anti-Rat IL-10

Alternative Name: Il10; Interleukin-10; CSIF; Cytokine synthesis inhibitory factor
Reactivity: Rat (QC Testing)
Isotype: Mouse IgG2b, κ
Immunogen: Recombinant Rat IL-10
Application: Intracellular block/flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_395691
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunoflourecent Staining and Flow Cytometric Analysis : The PE-conjugated A5-4 antibody (Cat. No. 555088) can be used for multicolor immunofluorescent staining and flow cytometric analyses to identify and enumerate IL-10-producing cells within mixed cell populations (See Figure). For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated (≤ 0.5 µg mAb/million cells). For specific methodology, please visit our web site, w ww.bdbiosciences.com , and go to the protocols section or the chapter on intracellular staining in the Immune Function Handbook. An appropriate isotype control is Cat. No. 555058. A useful control for demonstrating specificity of staining is either of the following: 1) preblock the conjugated A5-4 antibody with a ligand (e.g., recombinant rat IL-10; Cat. No. 555113) prior to staining, or 2) pre-block the fixed/permeabilized cells with unconjugated A5-4 antibody prior to staining. The staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. An isotype control should be used at the same concentration as the antibody of interest.