BD, 555214, BD OptEIA™ TMB Substrate Reagent Set

Application: ELISA (Tested During Development)
Regulatory Status: RUO
RRID: AB_2869044
Description: Description The TMB substrate reagent set is designed for use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is a colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.
Preparation And Storage: Preparation And Storage Store unopened reagents at 2-8°C. Do not use after the expiration date. Discard any remaining working solutions after use. Reagents should be brought to room temperature (20-25°C) prior to use. Allow at least 30 minutes for this process. Avoid prolonged exposure to light, contact with metal, air or extreme temperatures as color may develop.
Recommended Assay Procedures: Recommended Assay Procedures Prepare a working concentration of TMB substrate solution within 15 minutes prior to use by mixing equal volumes of Substrate Reagent A and Substrate Reagent B (e.g. for one 96-well plate, a 12 mL TMB substrate working solution can be prepared by mixing 6 mL of Substrate A with 6 mL of Substrate Reagent B).  When mixed together, TMB substrate solution should be colorless to a very faint blue. In the presence of peroxide-labeled conjugates, the solution should develop a definitive blue color. After the addition of sulfuric or phosphoric acid to stop the reaction, the color changes from blue to yellow. Danger: Substrate Reagent B (component 51-2607KC) contains 33.05% methanol (w/w), 13.34% glycerin (w/w), and 1.745% DMSO (w/w). Hazard statements Flammable liquid and vapour. Toxic if swallowed, in contact with skin or if inhaled. Causes damage to the central nervous system. Route of exposure: Oral. Causes damage to organs. Precautionary statements Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Keep container tightly closed. Ground and bond container and receiving equipment. Use explosion-proof [electrical/ventilating/lighting/] equipment. Use non-sparking tools. Take action to prevent static discharges. Use suitable extinguishing media to extinguish. Wear protective gloves / eye protection. Wear protective clothing. Do not breathe mist/vapours/spray. Use only outdoors or in a well-ventilated area. Wash thoroughly after handling. Do not eat, drink or smoke when using this product. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower.Take off immediately all contaminated clothing. Wash contaminated clothing before reuse. IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. IF SWALLOWED: Call a POISON CENTER/doctor/ if you feel unwell. Rinse mouth. IF exposed or concerned: Call a POISON CENTER/doctor/ Store in a well-ventilated place. Keep cool.  Store locked up.  Keep container tightly closed. Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.
Product Notices: Product Notices Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Description : Quantity/Size : Part Number : EntrezGene ID
N/A : 300.0 : N/A : N/A