BD, 555370, BD Pharmingen™ Purified Mouse Anti-Human CD9

Alternative Name: CD9 antigen (p24); 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; MRP-1; TSPAN29
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Human C-ALL Cells
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Western blot (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 0.5 mg/ml
Workshop Number: III 617
RRID: AB_395772
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures This reagent is effective for indirect immunofluorescence staining of human cells for flow cytometric analysis, western blotting with human platelets lysate at 2-4 µg/ml (in-house data), and immunohistochemistry on frozen tissue (spleen/thymus) sections at 2-10 µg/ml (in-house data). However, we do not recommend using this antibody on the formalin fixed paraffin tissue sections.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.