BD, 555857, BD Pharmingen™ Purified Mouse Anti-Human IgE

Alternative Name: Immunoglobulin E; IGHE; IGHε; Ig epsilon chain constant region
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a, κ
Application: ELISA (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_396179
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures For current recommendations of pairing mAbs to be used in two-site quantitative ELISA, please refer to the product information sheet under "ELISA and ELISPOT" at our website: http://www.bdbiosciences.com/us/s/resources.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.