BD, 555896, BD Pharmingen™ Purified Rat Anti-Human CD132

Alternative Name: IL-2RG; IL-2Rγ; γc; Common γ chain; Common gamma chain; gamma c; SCIDX1
Reactivity: Human (QC Testing), Dog (Tested in Development)
Isotype: Rat IgG2b, κ
Immunogen: Human γc Transfected Cell Line
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
Workshop Number: VI C-89
RRID: AB_396208
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures This reagent is effective for indirect immunofluorescence staining of human tissue for flow cytometric analysis. For flow cytometric applications, a three step labelling procedure is recommended for amplifying signal. Suggested protocol for 3-step staining using Lysed Whole Blood method: 1. Incubate 100 µl whole blood with primary (unconjugated) antibody for 20-30 minutes at room temperature. 2. Add 2 mls of 1X Pharm Lyse (10X Pharm Lyse, Cat. No. 555899) and incubate for 10-15 minutes. Centrifuge and aspirate. 3. Wash once with PBS/0.1% sodium azide/ 1% heat-inactivated fetal bovine serum (PBS-FBS). Centrifuge and aspirate. 4. Add biotinylated mouse anti-rat Ig's (Cat. No. 553883) and incubate for 20-30 minutes at room temperature. 5. Wash once with PBS-FBS. Centrifuge and aspirate. 6. Add SAV-PE (Cat. No. 554061) and incubate for 20-30 minutes in the dark at room temperature. 7. Wash once with PBS-FBS. Centrifuge and aspirate Resuspend in 0.5 ml of PBS-FBS and analyze by flow cytometry.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.