BD, 555991, BD Pharmingen™ Purified Mouse Anti-Human CD195

Alternative Name: CCR5; C-C chemokine receptor type 5; CC-CKR-5; CKR5; CHEMR13
Reactivity: Human (QC Testing)
Isotype: Mouse C57BL/6 IgG2a, κ
Immunogen: Human CCR5 Transfected Cell Line
Application: Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
Concentration: 0.5 mg/ml
Workshop Number: VII 70307
Entrez Gene ID: 1234
RRID: AB_396277
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunophenotyping studies of chemokine receptors need to be performed on freshly collected whole blood (<24 Hrs). Incubation with the antibody should be done at room temperature in the dark. Cellular manipulation, such as Ficoll-Paque™ separation, freezing, or exposure to cold temperatures prior to staining have been shown to cause a decrease in staining intensity and inconsistent results.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. An isotype control should be used at the same concentration as the antibody of interest. Ficoll-Paque is a trademark of Amersham Biosciences Limited. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.