BD, 556028, BD Pharmingen™ FITC Mouse Anti- BrdU Set

Reactivity: Human (QC Testing)
Application: Flow cytometry, Intracellular staining (flow cytometry) (Routinely Tested)
Regulatory Status: RUO
RRID: AB_396304
Description: Description Reacts with bromodeoxyuridine (BrdU) incorporated in place of thymidine during the S-phase of cycling cells. This technique enables measurement of the frequency of DNA-synthesizing cells in studies of cell activation and proliferation.
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Note: this product is routinely tested on fixed and permeabilized REH cell line with 70-80% cold ethanol. Please see the detail protocol at http://www.bdbiosciences.com/pharmingen/protocols/BrdU_Incorporation.shtml This product is NOT recommended to use with BrdU flow kits, e.g. Cat. No. 559619.