BD, 556350, BD Pharmingen™ Purified Mouse Anti- Calcineurin

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Human β1 isoform of calcineurin A Subunit aa. 349-505
Application: Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
Target Molecular Weight: 61 kDa
Concentration: 0.5 mg/ml
RRID: AB_396379
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include immunoprecipitation (1-2 µg/ml) and western blot analysis (1-2 µg/ml). Jurkat human T cells (ATCC TIB-152)2, HeLa human carcinoma cells (ATCC CCL-2), and A-431 human epidermal carcinoma cells (ATCC CRL-1555) are suggested as positive controls. A431 cell lysate (Cat. No. 611447) and HeLa cell lysate (Cat. No. 611449) are also available as ready-to-use positive western blot control.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.