BD, 556373, BD Pharmingen™ Biotin Mouse Anti-Human CD178

Alternative Name: Fas ligand; FASL; FASLG; CD95 ligand; CD95L; CD95-L; TNFSF6; APTL; APT1LG1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Mouse T lymphoma cells (L5178Y) expressing human FasL
Application: Flow cytometry (Routinely Tested), Western blot (Not Recommended)
Concentration: 0.5 mg/ml
RRID: AB_396394
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures Biotin-conjugated NOK-1 (Cat. No. 556376) is recommended for flow cytometry (titrate between 0.06 to 1 µg/1x10^6 cells). Biotin-conjugated NOK-1 is also available in a 100 test size (Cat. No. 556374), and has been optimally titrated for use in flow cytometry. NOK-1 has also been used for immunoprecipitation (see purified format, Cat. No. 556372), and to neutralize the cytotoxic activity of FasL. The NA/LE™ format of NOK-1 (Cat. No. 556371) should be used for all functional assays. NOK-1 and a related human FasL clone, NOK-2 (purified, Cat. No. 556376; BD NA/LE™, Cat. No. 556375) may give different profiles in neutralization assays. It is thought that NOK-1 and NOK-2 recognize different FasL epitopes. Neither NOK-1 and NOK-2 are suggested for western blot analysis. Clone G247-4 (Cat. No. 556387) is suggested for western blot.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.