BD, 556602, BD Pharmingen™ Purified Mouse Anti-NF-ATc1

Reactivity: Human (QC Testing), Mouse (Reported)
Isotype: Mouse IgG1, κ
Application: Western blot (Routinely Tested), Gel shift, Immunofluorescence, Immunoprecipitation (Tested During Development)
Target Molecular Weight: 100-120 kDa
Concentration: 0.5 mg/ml
RRID: AB_396478
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures 7A6 may be used for western blot analysis of NF-ATc1 (1-2 µg/ml). Note that while the predicted M W of NF-ATc is ~100 kDa, the observed M W may range between 100 to 120 kDa due to post-translational modification of NF-ATc.  Other applications include immunoprecipitation (2 µg/ml), mobility shift assays (EMSA) and immunofluorescence microscopy of paraformaldehyde-fixed, tissue cultured cells.  Jurkat human T cells (ATCC TIB-152) are suggested as a positive control for expression of NF-ATc.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.