BD, 556970, BD Pharmingen™ Purified Mouse Anti-Rat CD3

Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgM, κ
Immunogen: F344 rat splenic T cells stimulated with PMA plus A23187
Application: Flow cytometry (Routinely Tested), Cytotoxicity, Fluorescence microscopy, Immunohistochemistry-frozen, Immunoprecipitation, Stimulation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_396542
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures We recommend FITC- or PE-conjugated anti-mouse IgMa mAb DS-1 (Cat. No. 553516 or 553517, respectively) as a secondary antibody for immunofluorescent staining because we have found that polyclonal anti-mouse Ig antibodies may lose much of their reactivity to mouse IgM after adsorption with rat Ig. For IHC, we recommend the use of purified G4.18 mAb in our special formulation for immunohistochemistry, Cat. No. 550295. Although mAb 1F4 is reported to be effective for in vitro T-cell stimulation, we have found that the alternate anti-rat CD3 antibody G4.18 (Cat. no. 554829), soluble or immobilized, has greater stimulatory activity.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.