BD, 557273, BD Pharmingen™ Purified Mouse IgG1, κ Isotype Control

Isotype: Mouse BALB/c IgG1, κ
Application: ELISA, Flow cytometry, Isotype control (Routinely Tested), Immunohistochemistry (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_396613
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures This antibody is useful as a standard in ELISA or as an isotype-matched negative control for immunostaining. An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry). For immunohistochemical staining, we recommend the use of purified MOPC-31C mAb in our special formulation for immunohistochemistry (Cat. No. 550878). We recommend purified mouse IgG1 κ monoclonal antibody MOPC-21 (Cat. No. 555746/554121) for immunofluorescent staining of human whole blood and Cat. No. 556648 for non-human primate cells.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.