BD, 557393, BD Pharmingen™ Purified Hamster Anti-Mouse CD28

Reactivity: Mouse (QC Testing)
Isotype: Syrian Hamster IgG2, λ1
Immunogen: Mouse EL-4 (T-cell lymphoma) Cells
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), (Co)-stimulation, Blocking, Cytotoxicity, Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394764
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Recommended Assay Procedure: Precautions for flow cytometry: For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to identify T lymphocytes and/or NK cells and that purified anti-mouse CD16/CD32 mAb 2.4G2 [Mouse BD Fc Block™] (Cat. No. 553141) be used. Since this antigen is expressed at low density on resting peripheral T lymphocytes, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a "bright" third-step reagent, such as streptavidin-PE (Cat. No. 554061).  If Mouse BD Fc Block™ is used, it is important that the second-step anti-hamster IgG antibody does not cross-react with the Mouse BD Fc Block™.  Biotinylated anti-Syrian hamster IgG2 mAb G192-3 (Cat. No. 554029) would be a suggested second-step antibody to consider. Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals.  Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer.  Since endotoxin may also effect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.