BD, 557984, BD Pharmingen™ Alexa Fluor® 700 Hamster anti-Mouse CD3e

Alternative Name: CD3; CD3 epsilon; Cd3e; CD3ε; T3e
Reactivity: Mouse (QC Testing)
Isotype: Syrian Hamster IgG2, κ
Immunogen: Mouse T-cell receptor
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_396972
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed.
Recommended Assay Procedures: Recommended Assay Procedures Note: It has been observed that pre-incubation of thymus cell suspensions at 37°C for 2 to 4 hours prior to staining enhances the ability of anti-CD3e and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes. Note: Alexa Fluor® 700 conjugates of the hamster IgG2 isotype reportedly can non-specifically stain myeloid cells from mouse bone marrow.