BD, 558250, BD Pharmingen™ Purified Rat Anti-Mouse C3a

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Application: ELISA Capture (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_397067
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Purified I87-1162 as capture antibody can be paired with the biotinylated I87-419 anti-mouse C3a (Cat. No. 558251) as the detection antibody, using purified mouse C3a protein as standard. The purified I87-1162 antibody should be titrated between 1-4 µg/ml to determine the optimal ELISA plate-coating concentration. For plate coating, we recommend to use a pH 6.5 phosphate buffer. To obtain linear standard curves, doubling dilutions of purified mouse C3a protein (Cat. No. 558618), ranging from 781 pg/ml to 50 ng/ml are recommended for inclusion in each ELISA plate. Addition of FUT-175 (Futhan) to plasma samples at the time of sample collection provides additional protection from ex-vivo activation, and therefore ensures more accurate measurements that reflect the circulating levels of complement activation products.  For specific methodology, see Chapter 7: ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors. 2003. Techniques for Immune Function Analysis Application Handbook 1st Edition . BD Biosciences. This ELISA antibody pair shows no cross-reactivity with the following: recombinant mouse C5a, native human C3a, C4a and C5a, and rat C5a. This ELISA antibody pair shows a limited cross-reactivity with rat C3a at ~0.2% as compared to the corresponding mouse signal.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.