BD, 558307, BD™ Cytometric Bead Array (CBA) Rat IL-4 Flex Set

Assay Range: 40-10,000 pg/mL
Reactivity: Rat (QC Testing)
Regulatory Status: RUO
RRID: AB_2869151
Description: Description The BD™ CBA Rat IL-4 Flex Set is a bead-based immunoassay capable of measuring rat interleukin-4 (IL-4) in serum and cell culture supernatant samples. Rat reactivity was determined by testing samples with the BD CBA Rat IL-4 Flex Set. The biology and function of IL-4 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266 or 558267).
Preparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 10,000 pg/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.
Recommended Assay Procedures: Recommended Assay Procedures The BD CBA Rat IL-4 Flex Set must be used in conjunction with a BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266, 100 tests, or 558267, 500 tests), a flow cytometer, and FCAP Array™ Software.  Detailed instructions on the use of this product can be found in the manual for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Rat IL-4 Flex Set will be 10,000 pg/mL. An example standard curve is shown in figure 1. When multiplexing the BD CBA Rat IL-4 Flex Set assay with the BD CBA Rat IFN-γ Flex Set assay (Cat. No. 558305), significantly higher background will be seen in the rat IL-4 assay. This increased background, while reducing the sensitivity of the rat IL-4 assay, will not effect the quantitation of rat IFN-γ or IL-4 in any other way. The BD CBA Rat IL-4 Flex Set should not be used in the same assay well with any non-BD CBA Rat Soluble Protein Flex Set reagents (such as BD CBA Human or Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Rat Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 3.4 pg/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/mL, n =30) + 2 standard deviations.