BD, 558736, BD Pharmingen™ Purified Rat Anti-Mouse CD31

Alternative Name: Platelet endothelial cell adhesion molecule; Pecam1; PECAM-1; Pecam-1
Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgG2a, κ
Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D
Application: Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed, Immunoprecipitation (Reported), Immunohistochemistry-formalin (antigen retrieval required), Western blot (Not Recommended)
Concentration: 0.5 mg/ml
RRID: AB_397095
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures For IHC, we recommend the use of purified alternative anti-mouse CD31 mAbMEC 13.3 in our special formulation for immunohistochemistry, Cat. No. 550274.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.