BD, 558754, BD Pharmingen™ PE Hamster Anti-Mouse CD27

Reactivity: Mouse (QC Testing), Rat (Tested in Development)
Isotype: Armenian Hamster IgG1, κ
Immunogen: Armenian hamster fibroblast line ARHO12 transfected with mouse Cd27 cDNA
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_397106
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures This antibody conjugate is compatible with intracellular staining protocols using the BD Cytofix/Cytoperm™ Kit (Cat. No. 554714).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.