BD, 558905, BD Pharmingen™ FITC Mouse Anti-Human Granzyme A Reagent Set

Reactivity: Human (QC Testing)
Application: Flow cytometry (Routinely Tested)
Regulatory Status: RUO
RRID: AB_397156
Description: Description The primary mechanism by which cytotoxic T cells eliminate virally infected cells is by granule exocytosis. The release of cytotoxic granule contents by cytotoxic T lymphocytes (CTL) triggers apoptotic target cell death. CTL granules contain a pore-forming protein, perforin, and a group of serine proteases called granzymes. In the classic model, perforins create holes in the target cell membrane, allowing entrance of the granzymes. Granzyme A and B are the predominant granzymes activated after CTL activation, but each act via an independent apoptotic pathway; granzyme B is activated immediately, while granzyme A acts hours later. Granzyme B does not induce cleavage of caspase-3, lamin B, rho-GTPase or PARP, but does cleave DNA-PKcs and nuclear mitotic apparatus protein (NuMA). The physiological substrates for granzyme A in the apoptotic pathway have not been identified. Studies involving mice which are deficient in both granzyme A and B suggest a model whereby the granzyme B pathway may have evolved as the major apoptotic pathway with the granzyme A pathway acting as a backup. However, further research is needed to delineate the components of the distinct pathways. Clone CB9 recognizes human granzyme A. Purified human granzyme A was used as immunogen. Clone MOPC-21 is a mouse IgG1 isotype (negative) control. The MOPC-21 antibody has unknown specificity.  CB9 and MOPC-21 FITC conjugates are optimized in F/P ratios. The antibodies are routinely tested in parallel by flow cytometry. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Description : Quantity/Size : Part Number : EntrezGene ID
N/A : 100.0 : N/A : N/A