BD, 558934, BD Pharmingen™ PE Mouse Anti-Human CD119

Alternative Name: IFN- gamma receptor alpha chain
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Human IFN-γRα
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
Workshop Number: VI C-110
RRID: AB_397163
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent staining and flow cytometric analysis: PE Mouse Anti-Human CD119 (Cat. No. 558934) can be used for the immunofluorescent staining (≤ 1 µg antibody/10[6] cells) and flow cytometric analysis of the levels of membrane IFN-γRα expressed by human cell lines or human lymphoid cells. An appropriate R-PE conjugated immunoglobulin isotype control is the PE Mouse IgG1, κ Isotype Control (Cat. No. 554680). Since GIR-208 is a neutralizing antibody, it competes with IFN-γ for binding to its receptor. Therefore, the use of the GIR-208 antibody for immunofluorescent staining and flow cytometric analysis in systems where the natural ligand of the receptor is present may give an underestimation of IFN-γRα chain expression. Based on our testing results, the presence of recombinant human IFN-γ protein at levels above 200 ng/ml is sufficient to completely inhibit the binding of the GIR-208 antibody (at 0.06 µg/10[6] cells). In such cases, it is recommended that the investigator uses a non-neutralizing antibody to detect IFN-γRα chain expression by flow cytometry, such as PE Mouse Anti-Human CD119 (Cat. No. 558937). WB: The GIR-208 antibody has been reported to be useful for Western blotting.  Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.