BD, 558935, BD Pharmingen™ Purified Mouse Anti-Human CD119

Alternative Name: IFN-γ Receptor α; IFN-γRα; IFNGR1; IFNGR; IFN-gamma receptor 1/IFN-gamma-R1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2b, κ
Immunogen: Human IFN-γRα
Application: Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_397164
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent staining and flow cytometric analysis : Purified Mouse Anti-Human CD119 (Cat. No. 558935) can be used for the immunofluorescent staining (≤ 1 µg/ 10[6] cells) and flow cytometric analysis of the levels of membrane IFN-γRα expressed by human cell lines or human lymphoid cells. It is recommended that the purified format of this antibody be used in conjunction with Biotin Goat Anti-Mouse Ig (Multiple Adsorption)(Cat No. 553999) and PE Streptavidin (Cat No. 554061) in a three-layer staining procedure to amplify immunofluorescent signals.  An appropriate purified immunoglobulin isotype control is Purified Mouse IgG2b κ Isotype Control (Cat No. 555740). Since the GIR-94 is a non-neutralizing antibody, it can be used for immunofluorescent staining and flow cytometric analysis in systems where the IFN-γ ligand is present. Based on our testing results, the presence of recombinant human IFN-γ protein at levels below 1 µg/ml is insufficient to inhibit the binding of GIR-94 (at 0.06 µg mAb/1 million cells).