BD, 559050, BD Pharmingen™ Purified Mouse Anti-Human CD62L

Alternative Name: L-selectin; LECAM-1; SELL; LNHR; LSEL; LAM-1; LEU8; PLNHR; TQ-1; MEL-14
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Human Peripheral Blood T Lymphocytes
Application: Flow cytometry (Routinely Tested), Functional assay, Western blot (Tested During Development)
Concentration: 1.0 mg/ml
Workshop Number: V S059
RRID: AB_397186
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Functional Studies: Anti-LECAM-1 monoclonal antibodies have been shown to inhibit neutrophil localization at sites of acute inflammation. Anti-LECAM-1 monoclonal antibody (clone SK11) also induces suppressor function in CD4+ T lymphocytes but does not induce lymphocyte proliferation. Immunoblotting: Using anti-LECAM-1 monoclonal antibody (clone SK11) in Western blot analysis of extracts from Leu- 8-transfected COS cells and the human T-cell leukemia line (Jurkat) revealed at least seven species of similar molecular weight and at least two closely spaced bands of 76 kD, the largest molecular weight form seen in COS cells.
Product Notices: Product Notices Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.