BD, 559750, BD Pharmingen™ Biotin Rat Anti-Mouse Ig, κ Light Chain

Reactivity: Mouse (QC Testing)
Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Immunogen: Mouse IgG2b κ secreted by MPC-11 plasmacytoma
Application: ELISA (Routinely Tested), Flow cytometry (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_397314
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures ELISA: This antibody has been tested by ELISA (2 µg/ml). For the sandwich mouse IgG1, G2a, G2b, G3, IgM, IgA, and IgE ELISA, biotin-conjugated mAb 187.1 (cocktailed with biotin-conjugated anti-mouse Ig λ1, λ2, and λ3 light chain, R26-46 mAb, Cat. No. 553433) is optimal for detection with anti-mouse Ig isotype specific mAbs (A85-3, Cat. No. 553445; R19-15, Cat. No. 553387; R12-3, Cat. No. 553392; R2-38, Cat. No. 553404; II/41, Cat. No. 553435; C10-1, Cat. No. 556960; C10-3, Cat. No. 556969; R35-72, Cat. No. 553413) for capture, respectively. Immunofluorescent staining and flow cytometry : Biotinylated 187.1 mAb may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic Ig, κ light chain we recommend FITC-conjugated mAb 187.1 (Cat. No. 550003).