BD, 559812, BD Pharmingen™ PE Mouse Anti-Pig IFN-γ

Reactivity: Pig (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Recombinant pig IFN-γ protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_397341
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures IF/Flow: For immunofluorescent staining and flow cytometric analysis, the P2G10 antibody has been found useful to identify and enumerate IFN-γ producing cells within mixed cell populations. PE- conjugated P2G10 antibody (Cat. No. 559812) is especially suitable for these studies. For specific methodology, please visit the online protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. A useful control for demonstrating specificity of staining is either of the following: 1) pre-block the fluorochrome-conjugated P2G10 antibody with ligand prior to staining, or 2) pre-block the fixed/permeabilized cells with unlabeled P2G10 antibody (Cat. No. 559961) prior to staining. The intracellular staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgG1, κ isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized porcine cells is PE-MOPC-21 immunoglobulin (Cat. No. 554680); use at comparable concentrations to antibody of interest (eg, ≤ 0.5 µg mAb/1 million cells). ELISA Capture: The purified P2G10 antibody (Cat. No. 559961) is useful as a capture antibody for a sandwich ELISA for measuring porcine IFN-γ protein levels. Purified P2G10 antibody can be paired with the biotinylated P2C11 antibody (Cat. No. 559958) as the detecting antibody.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.