BD, 560268, BD Pharmingen™ Purified NA/LE Rat Anti-Mouse IL-17A

Alternative Name: IL-17; Il17; IL-17A; Il17a; CTLA-8; Ctla8; Interleukin-17A; interleukin 17A
Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgG1, κ
Immunogen: Recombinant Mouse IL-17A Protein
Application: ELISA Capture (Routinely Tested), Intracellular staining (flow cytometry), Neutralization (Tested During Development)
Concentration: 1.0 mg/ml
RRID: AB_1645260
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures ELISA: Purified TC11-18H10 antibody (Cat. No. 560268) can be useful as a capture antibody for sandwich ELISA measuring mouse IL-17A protein levels.  Purified TC11-18H10 antibody can be paired with biotinylated TC11-8H4 (Cat. No. 555067) as the detector antibody, with recombinant mouse IL-17A protein as the standard.  Investigators are encouraged to titrate purified TC11-18H10 from 2.0 µg/mL - 0.5 µg/mL for optimal concentration determination for ELISA capture.  To obtain linear standard curves, doubling dilutions of recombinant mouse IL-17A are suggested from 2,000 - 15 pg/mL.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.