BD, 560619, BD Pharmingen™ APC Mouse Anti-Human CD196 (CCR6)

Alternative Name: BN-1; C-C CKR-6; C-C chemokine receptor type 6; CC-CKR-6; CCR-6
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Mouse IgG1, κ
Immunogen: Human CD196/CCR6 Peptide
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 5 µl
Workshop Number: IX 48
RRID: AB_1727439
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Recommended Assay Procedures: Recommended Assay Procedures Flow cytometry: Chemokine receptors are known to internalize during manipulation resulting in low frequency expression.  Investigators are advised to perform immunophenotyping studies of chemokine receptors on freshly collected samples (<24 Hrs). Incubation with the antibody should be done at 4°C in the dark. Cellular manipulation, such as Ficoll separation, freezing, or exposure to cold temperatures prior to staining should be minimized and have been shown to cause a decrease in staining intensity and/or inconsistent results. Investigators should note that alternative staining procedures may be neccessary.  A multiple-step staining procedure may be helpful, in some instances, to amplify immunofluorescent signals for the flow cytometric analysis of CD196 expression.  Investigators may find the Purified Mouse Anti-Human CD196 antibody (Cat. No. 559560) to be useful in conjunction with appropriate secondary and tertiary reagents for detecting low frequency expression, such as with Biotin Goat Anti-Mouse Ig (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061) or APC Streptavidin (Cat. No. 554067).
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.