BD, 560634, BD Pharmingen™ APC Rat Anti-Mouse CD14

Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Immunogen: Recombinant Mouse CD14
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_1727426
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Recommended Assay Procedures: Recommended Assay Procedures Flow Cytometry: Investigators should note that Mouse BD Fc Block™ purified rat anti-CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) and antibodies of the rat IgG2b isotype may potentially interfere with the reactivity of the APC Rat Anti-Mouse CD14 antibody (clone rmC5-3) in a concentration-dependent manner. For alternative methods for inhibition of non-specific reactivity, investigators may find the use of purified mouse IgG at a 10-100-fold excess to be more appropriate.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.