BD, 561198, BD Pharmingen™ PE Mouse anti-Human IL-17F

Alternative Name: Interleukin-17F; ML-1; cytokine ML-1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Human IL-17F
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_10611873
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix/Cytoperm™ reagents, BD Pharmingen™ Human FoxP3 Buffer Set or BD™ Phosflow fixation and permeabilization buffers (Fix buffer I with Perm/Wash Buffer I, Perm Buffer II, or Perm Buffer III).
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Cy is a trademark of GE Healthcare. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.