BD, 561609, BD Pharmingen™ Mouse Naive/Memory T Cell Panel

Reactivity: Mouse (QC Testing)
Application: Flow cytometry (Routinely Tested)
Regulatory Status: RUO
RRID: AB_2869387
Description: Description Note: The panel is 50 test size. Extra material for each component for an additional 10 tests is provided for instrument set up purposes. The antibodies are supplied in an aqueous buffered solution containing BSA, protein stabilizer, and ≤ 0.09% sodium azide. Multicolor immunofluorescent staining followed by flow cytometric analysis and sorting has led to the phenotypic and functional characterization of multiple peripheral T cell subsets. Three major T cell subsets have been well characterized, i.e., naïve, memory and effector T cells. Naïve mouse T cells have a relatively homogenous cell surface phenotype. They express high levels of the peripheral lymph node homing receptor, CD62L (L-selectin), and low to intermediate levels of the adhesion molecule, CD44 (Pgp-1). Effector T cells coexpress variable to low levels of CD62L and high CD44 levels whereas memory T cells coexpress variable to high levels of CD62L and high levels of CD44. The Mouse Naive/Memory T Cell Panel contains fluorescent antibodies (each optimized at 5 μl per test) that are specific for the cell surface antigens: CD44, CD62L, CD4 and CD3. The panel was designed to standardize the multicolor staining and flow cytometric characterization of the three major CD4+ T cell subsets that arise as a consequence of development or clonal expansion and differentiation driven by antigenic stimulation (eg, in response to allergens, infectious disease or vaccination). This fluorescent antibody panel was flexibly designed to permit the researcher's incorporation of FITC- or Alexa Fluor® 488-conjugated antibodies/probes or cells expressing green fluorescent proteins into flow cytometric analysis of mouse T cell subsets.
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. The antibody was conjugated with APC-Cy7 under optimum conditions, and unconjugated antibody and free APC-Cy7 were removed.