BD, 561769, BD Pharmingen™ FITC Rat Anti-Mouse CD21/CD35

Alternative Name: CR2/CR1
Reactivity: Mouse (QC Testing)
Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
Immunogen: Purified Mouse CR1
Application: Flow cytometry (Routinely Tested), Fluorescence microscopy (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395070
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures We have observed that the staining intensity of FITC-conjugated 7G6 mAb is severely reduced after fixation of stained leukocytes (≤ 1 hour with 1% formaldehyde). Therefore, one should not fix the stained cells prior to flow cytometry. We have found that freshly-isolated leukocytes and cell lines may wait for analysis in wash buffer at 4°C, without fixation, for up to 18 hours post-staining without loss of viability. Activated lymphocytes may lose viability rapidly, and data should be collected within 5 hours post-staining.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.