BD, 563846, BD Horizon™ BV421 Goat Anti-Mouse Ig

Reactivity: Mouse (QC Testing)
Isotype: Goat Ig
Application: Flow cytometry (Routinely Tested), Bioimaging, Immunofluorescence, Immunohistochemistry (Tested During Development)
Concentration: 0.2 mg/ml
RRID: AB_2738449
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Recommended Assay Procedures: Recommended Assay Procedures For confocal microscopy systems, a 405 nm laser is the optimal excitation source with optimal emission collection centered at 421 nm. For epifluorescence microscopes with broad spectrum excitation sources, BD Horizon BV421 can be visualized using standard configurations for DAPI or Pacific Blue. The recommended optimal excitation and emission filters are 392/23 nm and 430/25 nm bandpass filters, respectively. For specific multicolor imaging applications, the exact filter configurations should be optimized by the end user. For additional instrument/filter configuration information, please visit http://www.bdbiosciences.com/research/cellularimaging For imaging applications suboptimal staining can sometimes occur on some nuclear targets. For immunofluorescence applications, suboptimal staining can sometimes occur with targets that localize to the nucleus or sub-nuclear compartments, such as Ki-67. In multicolor immunofluorescence applications, we recommend the use of BD Horizon™ BV480 for targets localized to the nucleus and the use of BV421 for cell surface and cytoplasmic targets.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR. DRAQ5™ is a registered trademark of BioStatus Ltd. Triton is a trademark of the Dow Chemical Company. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.