BD, 566922, BD Pharmingen™ PE Mouse Anti-Human Integrin αvβ6

Alternative Name: αVβ6; avb6; CD51/β6; ITGAV/ITGB6; Integrin alpha v beta 6
Reactivity: Human (QC Testing), Mouse (Reported)
Isotype: Mouse 129 x C57BL/6 IgG2a
Immunogen: 129/C57BL/6 β6 -/- Mice were mmunized with Human αvβ6 or Mouse Keratinocytes
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_2869950
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Note: The binding of the 10D5 antibody to αvβ6 is divalent-cation dependent. For optimal results, ensure ~ 1 mM Mg2+ is present in the staining and analysis buffers when using the 10D5 antibody for flow cytometric analysis. BD Pharmingen Stain Buffer (Cat. 554656/554657) contains Mg2+. BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.