Cytiva, 17531901, HisTrap™ FF columns for high-capacity protein purification

HisTrap FF
Prepacked Ni2+ Sepharose columns for histidine-tagged protein purification
• High binding capacity, approx. 40 mg/mL resin.
• Negligible leakage of Ni 2+ .
• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni 2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni 2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications
The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the resin with different metal ions for optimization of purification protocols.