Cytiva, E70092Y, Amersham Shrimp Alkaline Phosphatase

Amersham Shrimp Alkaline Phosphatase, 500 Alkaline phosphatase is suitable for dephosphorylation of 5' and 3'-overhangs, recessed ends, blunt ends, and single nucleotides. Following incubation, alkaline phosphatase can be easily inactivated by heating. • Bacterial alkaline phosphatase recombinantly expressed in E. coli . • Active in PCR and restriction endonuclease buffers. • Inactivated by heating at 75°C for 5 minutes. Use together with Exonuclease I for cleaning up PCR reactions prior to downstream application such as DNA sequencing. Unit Definition One unit is the amount of the enzyme required to dephosphorylate 1 µg of linearized pUC57 DNA termini in 10 minutes at 37°C.