Merck, 11093657910, DIG DNA Labeling and Detection Kit

DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of random oligonucleotides as primers. Contents: •Unlabeled Control DNA 1, 100 μg/ml •Unlabeled Control DNA 2, 200 μg/ml •DNA Dilution Buffer •DIG-labeled Control DNA, 5.2 μg/ml •10x Hexanucleotide Mix •10x dNTP Labeling Mixture •Klenow Enzyme, Labeling grade, 2 U/μl •Anti-digoxigenin-AP-conjugate, 750 U/ml •NBT/BCIP Concentrated Stock Solution •Blocking Reagent