Merck, 56403AST, apHera™ NH₂ HPLC Column

apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column. Features of Amino apHera™ Columns •High efficiency for carbohydrate analysis •Ideal for basic conditions/amphoteric detection •High selectivity mono- to oligosaccharides •pH range 2-13 •300 angstrom pore size •Stable, predictable retention •Long column life