Merck, 8.52044.0500, FMOC-Asn(Trt)-OH

High purity Fmoc-protected amino acid for research and process production of peptides, with very low levels of dipeptide, free-amino acids and acetic acid impurities. Fmoc-Asn(Trt)-OH has good solubility properties in most organic solvents, and its use has been shown to result in significantly purer peptides than other derivatives used for the introduction of Asn [1,2]. Coupling can be performed by standard procedures. The trityl group is normally removed by 95% TFA in 1-3 hours, with no alkylation of Trp residues. When Asn(Trt) is the N-terminal residue, the reaction time may need to be extended to ensure complete deprotection [3]. Associated Protocols and Technical Articles Fmoc-amino acids for Peptide Production Cleavage and Deprotection Protocols for Fmoc SPPS Literature references [1] P. Sieber, et al. in ′Innovation & Perspectives in Solid Phase Synthesis, 1st International Symposium′, R. Epton (Eds), SPCC UK Ltd., Birmingham, 1990, pp. 577. [2] P. Sieber, et al. (1991) Tetrahedron Lett., 32, 739. [3] M. Friede, et al. (1992) Pept. Res., 5, 145.