Merck, M8250-1KG, MES hydrate

MES hydrate, or 4-morpholineethanesulfonic acid hydrate, is a zwitterionic and morpholinic buffer renowned as one of the \Good′s\ buffer for biological and biochemical applications. Its outstanding buffering capacity in the pH range of 5.5 to 6.7 makes it versatile for a wide array of research applications. Notably, MES Buffer minimizes interaction with common metals used in environmental and biological studies, preventing interference with experimental outcomes. With excellent water solubility and virtual insolubility in lipids, MES Buffer is impermeable to membranes, ensuring efficacy in cellular environments. Its applications span maintaining stability in cell culture media, protein-based buffer formulations, and electrophoresis running buffers. MES Buffer may also find usage in purifying antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as a better alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers such as citrate and malate. MES Buffer proves valuable for adjusting growth media pH, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings.