Merck, RCI1MAG-87K-01, MILLIPLEX® Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay

Drug-induced cardiotoxicity can be life threatening and necessitates limiting an otherwise-effective treatment. In fact, cardiovascular toxicity has been reported to account for over 40% of drug candidate withdrawal in premarketing clinical trials. Many therapeutic agents have been reported to induce or exacerbate heart failure including anti-tumor medications, immunomodulating drugs, antidepressants, and calcium channel blocking agents to name a few. Conventional biomarkers of cardiotoxicity such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) have gradually been withdrawn due to low tissue specificity. The number of cardiac-specific biomarkers adapted from clinical studies of cardiotoxicity continues to rise. The reverse translational application of these auspicious biomarkers in experimental animal models could improve the power of detection for diagnosis, helping to predict the occurrence of cardiotoxicity in humans. Emerging biomarkers of cardiac injury include multiple categories of proteins: characteristic muscle cell proteins (cTnI, cTnT, FABP3 and MYL3), cytosolic kinase (CKM), secreted heparin-binding protein (FSTL1) and metalloprotease inhibitor (TIMP1). Numerous studies have demonstrated elevated levels of these biomarkers in experimental animal blood samples. In order to meet the increasing need to provide screening assays for experimental biomarkers of drug-induced cardiac injury in pre-clinical and translational research models, we have developed the MILLIPLEX® Rat Cardiac Injury Bead Panel 1. This configurable 7-plex panel is the most versatile system available for cardiac injury screening in pre-clinical and translational research models. The MILLIPLEX® Rat Cardiac Injury Bead Panel 1 (RCI1MAG-87K) contains all the components necessary to simultaneous quantify any or all of the following 7 analytes in serum and plasma samples: Cardiac Troponin I (cTnI), Cardiac Troponin T (cTnT), Creatine Kinase, Muscle (CKM), Fatty Acid Binding Protein 3 (FABP3), Follistatin-like Protein 1 (FSTL1), Myosin Light Chain 3 (MYL3), Tissue Inhibitor of Metalloproteinase-1 (TIMP-1). This kit uses a 96-well format, contains lyophilized standard cocktail and two quality controls, and can measure up to 38 serum or plasma samples in duplicate. Panel Type: Toxicity