Qiagen, 339411, Custom LNA Oligonucleotide (100 nmol synthesis)

100 nmol synthesis Custom LNA Oligonucleotide; various purification and labeling options available

Features

- Customize your own LNA oligonucleotides
- Use your own design or let QIAGEN's LNA experts design them for you
- Select from a wide range of labels and modifications
- Benefit from the easy-to-use online design tool

Product Details

Custom LNA Oligonucleotides are ideal for studies involving short or very similar sequences. The high affinity of an LNA-enhanced oligonucleotide to its complementary sequence results in dramatically improved specificity and sensitivity, when compared with traditional DNA or RNA oligos. In many cases, LNA-enhanced oligonucleotides can be used to distinguish between sequences differing by only a single nucleotide, a feature that can be critical for the success of many experiments. Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us! For Custom LNA Oligonucleotide Large Scale and Custom LNA Oligonucleotide Manual Design, please contact us for ordering and for further information, or email our Support Team directly.

Principle

- LNA will bind very tightly to other LNA residues. Avoid self-complementarity and cross-hybridization to other LNA-containing oligonucleotides
- Keep the GC content between 30–60%
- Avoid stretches of more than 4 LNA bases, except when very short (9–10 nucleotides) oligonucleotides are designed
- Avoid stretches of 3 or more Gs or Cs
- For novel applications, design guidelines may have to be established empirically

Use the guidelines below when designing your own Custom LNA Oligonucleotides:

Find more information about the LNA technology here and how to design LNA oligonucleotides here .

Applications

- miRNA research
- Small RNA research
- SNP genotyping
- mRNA antisense oligonucleotides
- Allele-specific PCR
- RNAi
- DNAzymes
- Fluorescence polarization probes
- Molecular beacons
- Microarray gene expression profiling
- Gene repair/exon skipping
- Splice variant detection
- Comparative genome hybridization (CGH)