Qiagen, 76743, miRCURY Exosome Cell/Urine/CSF Kit

For isolation of exosomes and other extracellular vesicles from cell culture supernatants, urine or CSF; includes 2 x 25 ml Precipitation Buffer B, 10 ml Resuspension Buffer

Features

- Excellent recovery of exosomes and other extracellular vesicles
- Easy and straightforward protocol that takes less than 2 hours
- No ultracentrifugation or phenol/chloroform steps required
- Fully compatible with the miRCURY LNA miRNA PCR System
- Suited for a variety of applications, such as miRNA or RNA profiling

Product Details

miRCURY Exosome Kits enable high-quality and scalable exosome isolation with an easy protocol that does not require special laboratory equipment. The miRCURY Exosome Serum/Plasma Kit is optimized for serum and plasma samples, while the miRCURY Exosome Cell/Urine/CSF Kit is designed for processing cell-conditioned media, urine and CSF samples. Both kits provide high exosomal recovery and seamless integration with different downstream assays.

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Performance

Efficient exosome isolation from serum and plasma

The miRCURY Exosome Serum/Plasma Kit is optimized for isolation of exosomes from serum and plasma samples. This kit includes thrombin for efficient isolation of exosomes from plasma. To evaluate the efficiency of exosome isolation, we quantified the fraction of exosomes found in the pellet versus the supernatant, which is normally discarded (see figure Exosome recovery from serum ). We observed a very high enrichment rate of extracellular vesicles.

Efficient exosome isolation from cell-conditioned media, urine and CSF

The miRCURY Exosome Cell/Urine/CSF Kit is optimized for isolation of exosomes from cell-conditioned media, urine and CSF samples. In studies comparing the fraction of exosomes in the pellet versus supernatant for these sample types, we observed a very high exosome enrichment rate (see figure Exosome recovery from cell-conditioned media and Exosome recovery from urine ). Typically, the amount of miRNA present in urine and CSF samples is very limited in healthy individuals. Therefore, we highly recommend performing an exosome isolation of these biofluids prior to RNA isolation and PCR profiling. This allows you to use the exosome isolation kit as a way of concentrating the sample, while keeping the amount of potential PCR inhibitors to a minimum (see figure Detect more miRNAs in biofluids using exosome isolation kits ).

Principle

- Immune regulation (e.g., tumor-derived exosomes may help tumors evade the immune response)
- Blood coagulation
- Cell migration
- Cell differentiation
- Cell-to-cell communication

Why study exosomes?