Qiagen, 9001910, QIAgility HEPA/UV (w/o PC), PrioPLUS

Instrument and service agreement package: robotic workstation for automated PCR setup (with UV light and HEPA filter) and QIAgility software; includes installation, application training, Full Agreement for three years with a two business day response time and three preventive maintenance visits. Does not include a laptop.
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Features

- Automated PCR setup in all formats
- Convenient, easy-to-use software
- Pre-optimized experiment setup
- Standardized results and increased productivity
- Elimination of manual pipetting steps

Product Details

The QIAgility is a compact benchtop instrument that enables rapid, high-precision setup of PCR experiments with ready-to-use software for ease and convenience. The software provides step-by-step guidance for worktable setup and automatic calculation of all mixes, eliminating the need to program pipetting steps. Just select the kit and cycler, define your targets and start the run. For added flexibility the QIAgility is operated via a laptop computer.

Explore the virtual demo to learn more about the QIAgility.

​QIAgility operates in conjunction with QIAgility Accessories and QIAgility Consumables .

Performance

The QIAgility fully automates proven QIAGEN PCR kits for use with various workflows, and offers pure pipetting precision, enhanced safety features, and exceptional reliability and reproducibility.

Unmatched pipetting precision with QIAgility

The QIAgility instrument operated by the QIAgility Setup Manager Software demonstrates exceedingly high accuracy and precision of dispensed volumes as verified by the Artel MVS system (see figure “ Exceptional pipetting precision and accuracy spanning over a broad volume range ”). These results indicate that the QIAgility can produce automated, high-performance liquid dispensing results, while avoiding common errors that can be associated with manual pipetting and thereby reducing the time to result.

Regardless of whether you pipet water or QIAGEN ready-made reaction mix to set up a PCR experiment, the QIAgility instrument can dispense volumes that are highly precise and also generate significantly reproducible replicates (see figure “ Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions ”).

High performance PCR setup using QIAgility

A QuantiNova SYBR Green PCR assay setup was performed manually and automated in parallel using the QIAgility along with its Setup Manager Software. The amplification run was done on the Rotor-Gene Q and resulting data analyzed using the Q-Rex Software. Consistent results were obtained from both manual and automated reaction setup using the same template dilutions (see figure “ Comparison of manual and automated assay setup using series dilutions ”).

Exceptional linearity of QIAgility

QIAgility exhibits excellent linearity and assures accurate and reliable use of sample for an assay. To assess the linearity of the system, pure gDNA was serially diluted using the QIAgility and measured for their concentrations on the QIAxpert. The quantifications from the QIAxpert were plotted against the known concentration values, and the resulting plot demonstrates the exceptional linearity (with a regression coefficient of >0.999) of QIAgility (see figure “ High system linearity of QIAgility ”).

Principle

Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that are typically prone to human error.

Liquid-handling tasks are performed by the single-channel pipetting head, which provides fast transfer of liquids with various types of plasticware. For example, 96 samples with the QuantiFast SYBR Green PCR Kit with 2 standard series can be setup in approximately 45 minutes. The worktable features a flexible layout and supports almost all kinds of cyclers and labware, giving high application versatility. Dynamic liquid-level sensing, facilitated by conductive filter tips offers maximum safety control.

The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. A built-in sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. The UV light ensures effective worktable decontamination and the HEPA filter provides positive clean air pressure throughout PCR setup.

QIAgility is a compact benchtop instrument with a very small footprint. It fits easily onto most lab benches and can be readily moved into biosafety level laboratories. For added flexibility, the QIAgility comes with a laptop computer. To reduce your work load, comprehensive run reports containing all run information are generated automatically during each protocol.

Applications

- Gene expression analysis
- Genotyping
- Cloning
- Pathogen detection
- Food and veterinary testing

The instrument is highly suited for laboratories performing PCR and RT-PCR reactions with applications in: ​

The new QIAgility Setup Manager Software offers a complete solution for qPCR setup, sample normalization, CE loading, certain liquid handling, as well as sample pooling. The software includes Gene Expression, Pathogen Detection, Sample Pooling and Generic PCR plug-ins. The Gene Expression and Pathogen Detection plug-ins are optimized for use with QIAGEN gene expression and pathogen detection kits, such as QuantiNova, miRCURY and RT² kits. The Generic PCR plug-in allows flexible qPCR setup, liquid handling and support for the whole HID workflow.

Check the instrument operating software and protocol files under product resources for more information.