Qiagen, 9003240, TissueLyser III

Bead mill, 100–120/220–240 V, 50/60 Hz; requires TissueLyser Adapters (available separately)

Features

- Convenient and secure disruption process
- Touch screen for ease of use
- Integrated software for pre-programmed and customizable protocols
- Wide range of accessories to process versatile sample volumes
- Reproducible results for difficult-to-lyse samples (e.g., cells, tissues, bones, etc.)

Product Details

The TissueLyser III simultaneously disrupts multiple biological samples through high-speed shaking in tubes or 96-well plates with stainless steel, zirconium, ceramic or glass beads. Using the appropriate adapter set, up to 192 samples can be processed at the same time. Alternatively, a grinding jar set can be used to process large samples. A range of beads and bead dispensers are also available.

The convenient touchscreen and rotary knob make it easier to run and customize disruption protocols. The TissueLyser III has 7 pre-programmed protocols and 5 customizable protocols for maximum sample versatility. The TissueLyser also allows you to create custom program cycles to conveniently set your disruption parameters.

TissueLyser II accessories , including adapter sets, are compatible with the TissueLyser III.

Performance

The TissueLyser III is well-suited for high-throughput disruption of animal and plant tissues, bacteria and yeast. Highly reproducible purification of high-quality DNA and RNA is achieved, even with difficult-to-lyse samples (see figures " Microbial sample types − DNA yield ", " Accurate display of microbial community OTUs from stool sample ", " Accurate display of alpha diversity from stool sample ", " Easy and effective lysis of plant samples for DNA extraction ", " Easy and effective lysis of plant samples for RNA extraction ", Easy and effective lysis of tissue samples for DNA extraction ", " Easy and effective lysis of tissue samples for RNA extraction " and " No cross-contamination in 96-well plates with the TissueLyser III ").

Principle

Instrument: Purpose
QIAcube Connect: Purification of DNA and RNA
QIAcube HT: 96-well plate format
QIAsymphony SP: 1–96 samples per run
EZ2 Connect: 1–24 samples per run

Analyte purified: Sample type
DNA: Human tissues
DNA: Animal tissues
DNA: Plant tissues
DNA: Microbial samples
RNA: Easy-to-lyse tissues
RNeasy Plus Kits
RNeasy Protect Kits
RNA: Fiber-rich tissues
RNA: All types of tissues
RNeasy Universal Tissue Kits
RNA: Plant tissues
RNA: Microbial samples
RNeasy PowerSoil Total RNA Kit
microRNA: All types of tissues

Efficient sample disruption is a prerequisite for nucleic acid purification procedures. Incomplete sample disruption can lead to significantly reduced yields and can increase the risk of clogging when using purification columns or magnetic particles. The TissueLyser III thoroughly disrupts and simultaneously homogenizes biological samples in the presence of a lysis buffer. Plant tissues can alternatively be disrupted and homogenized in the absence of a lysis buffer.

Tubes containing the sample and beads are placed in a TissueLyser Adapter Set and are shaken at a very high speed, causing the samples to be disrupted and homogenized by the beating and grinding effect of the beads. The tubes can be discarded after use to prevent cross-contamination and to save time, as there is no need to clean tubes after sample disruption. In addition, there is no cross-contamination during sample disruption as each tube is securely sealed with its own lid.

The TissueLyser III can also disrupt and homogenize large samples when used in combination with a Grinding Jar Set. Samples are placed in a 10 mL jar containing a grinding ball and shaken at high speed.

The TissueLyser III is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA and RNA. The TissueLyser III complements QIAGEN's automated solutions for high-throughput sample preparation and analysis (see table "QIAGEN high-throughput automation"), such as the QIAcube HT and QIAsymphony SP. This easy-to-use instrument automates purification of DNA and RNA from 1–192 samples. The TissueLyser III is also compatible with QIAGEN manual sample preparation kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems").

Procedure

Based on the adapter used during a run, the TissueLyser III can process up to 48 samples in 2 mL tubes or up to 192 samples in 96-well plates. Tubes and plates remain securely sealed during disruption to prevent cross-contamination. Depending on the sample type, disruption is carried out using stainless steel, zirconium, ceramic, or glass beads. The TissueLyser Bead Dispensers are available to conveniently deliver single beads into microcentrifuge tubes or to deliver 96 beads in parallel into collection microtubes. The TissueLyser III can also disrupt large samples when used in combination with Grinding Jar Sets.

Applications

The ability to process up to 192 samples per run makes the TissueLyser III the ideal front-end solution to access biological information for genomic, transcriptomic, and metatranscriptomic applications. For next-generation high-throughput sequencing technologies, the TissueLyser III is the disruption instrument of choice. A wide range of QIAGEN sample purification kits are compatible with the TissueLyser III, and sample purification can be performed manually or can be automated using the QIAcube Connect, QIAcube HT, QIAsymphony SP or EZ2 Connect.