Qiagen, 911001, QIAcuity One, 2plex Device

One-plate digital PCR instrument (cat.no 911000 QIAcuity One, 2plex instrument) for detecting up to 2 fluorescent dyes, roller, USB flash memory and QIAcuity Software Suite: includes 1 preventive maintenance visit. 1 year warranty on labor, travel, and parts also included.
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Features

- Fully integrated system
- Scalable format (1-, 4- and 8-plate instruments)
- Advanced multiplexing capabilities (up to 12 plex)
- Up to six standard channels plus two hybrid channels for LSS dyes
- Flexible sample throughput
- Comprehensive results in approx. 2 hours

Product Details

The QIAcuity Digital PCR System is designed to deliver precise and multiplexed quantification results for mutation detection, copy number variation (CNV), gene expression studies, gene-editing analysis, and many more. This nanoplate-based system seamlessly integrates a standard dPCR workflow of partitioning, thermocycling and imaging into a walk-away automated platform with minimal hands-on time.

The system is used in conjunction with the QIAcuity nanoplates, reagents and assays .

Explore the virtual demo to learn more about QIAcuity.

Performance

Feature: QIAcuity One 2plex
Plates processed: 1
Detection channels: 2
Multiplexing capability: 4
Thermocycler(s): 1
Time to result: Approx. 2 h
Throughput (samples processed in 8-hour shift, including overnight run): Up to 480 (96-well) Up to 120 (24-well)

The QIAcuity Digital PCR System makes absolute quantification accessible and affordable for all laboratories. The walk-away automation integrates and streamlines the entire digital PCR workflow of partitioning, thermocycling and imaging into a single instrument with minimal hands-on time. It is also easy to adapt your current qPCR assays to the QIAcuity Digital PCR System. No change in plate handling is required when coming from qPCR, assuring fast assay setup and quick results in approx. 2 hours.

QIAcuity Instruments – features and specifications

1) Requires use of QIAcuity High Multiplex Probe PCR Kit in case of multiplexing >5plex 2) Hybrid channels are used for Long Stokes Shift (LSS) dyes 3) Detection of 12 targets in parallel can be achieved by using amplitude multiplexing in the six standard channels. When combining hybrid channels for single plex LSS dyes and amplitude multiplexing, the total possible multiplex can be increased to 14. However, this combination is not recommended due to the optimization demand for all assays used in a reaction mix and their corresponding crosstalk compensation.

Principle

In just 3 simple steps, you can have the dPCR result you want in approx. 2 hours: pipette and load, run the experiment, analyze results.

The principle of the dPCR reaction in the nanoplates is described here .

Procedure

Just like in qPCR experiments, sample preparation includes the transfer of master mix, probes and primers to a 96- or 24-well nanoplate, followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single fully automated instrument that takes users from the sample to result in under 2 hours. One can perform analysis on the Software Suite, providing the concentration in copies per microliter of your target sequence as well as for quality control such as positive samples or NTC. This analysis can also be extended to remote computers within the same local area network (LAN).

Applications

- Rare mutation detection
- Copy number variation analysis
- Gene expression analysis
- Pathogen detection
- Genotyping
- miRNA research
- Cell and gene therapy

The QIAcuity instruments, together with the QIAcuity Nanoplates and the QIAcuity PCR kits, enable digital PCR applications, including: