Sartorius, 101000010, PULSin® Protein Delivery

The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.

PULSin® is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.Key Benefits:
Unique: One reagent for protein, antibody, and peptide delivery into mammalian cells.
Optimized: Efficient delivery in live primary cells and difficult to transfect cells.
Innovative: Easy to use alternative to chemical conjugation and viral transduction

Specifications
Applications:
Cell Type: Adherent & suspension cells grown in presence of serum

Biological Information:
Hepes dilution buffer: 20 mL
Molecule delivered: Protein, Antibody, Peptide
Number of transfections: 0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates
Positive control (R-phycoerythrin): 20 µg

General Specifications:
Amount of Reagent: PULSin® 0.4 mL
Reagents and Kits Type: PULSin® - Protein delivery

Product Information:
Provided With: Hepes Buffer (20mM) for protein dilution, R-Phycoerythrin used as a positive control

Storage Conditions:
Storage Conditions: 5 °C ± 3 °C