Sartorius, 101000025, jetOPTIMUS® DNA Transfection

jetOPTIMUS® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in adherent cells, even in hard-to-transfect cells.  In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume which preserves cell viability and morphology.

jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells resulting in higher transfection efficiency, even in hard to transfect cells. Tested on various primary cells and cancer cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression with minimal impact on cell viability and morphology.
jetOPTIMUS® is highly efficient for DNA delivery for various applications, such as transient and stable gene expression, CRISPR genome editing using DNA approach, as well as virus production in small scale.
Key Benefits
Highly efficient:Maximal gene expression in easy and hard-to-transfect cells .
Cost-effective:Minimum reagent volume and DNA quantity required .
Biologically relevant:Excellent cell viability & morphology .
Time-saving:Transfection with an optimized ready-to-use protocol

Specifications
Applications:
Application: Transient and stable gene expression from plasmid DNA transfection; CRISPR Genome editing using DNA approach; Small scale virus production
Cell Type: Adherent cells and hard-to-transfect cells; Primary cells (epithelial, hepatocyte, endothelial, fibroblast, etc…); Stem cells (embryonic, mesenchymal, induced pluripotent); Cancer cell lines; Neurons after fibroblast

Biological Information:
Buffer Size: 2 x 60 mL
Molecule delivered: Plasmid DNA
Number of transfections: 1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol

General Specifications:
Amount of Reagent: jetOPTIMUS® 0.75 mL
Reagents and Kits Type: jetOPTIMUS® - DNA transfection

Product Information:
Provided With: jetOPTIMUS® Buffer

Storage Conditions:
Storage Conditions: 5 °C ± 3 °C