Sartorius, 101000183, pPLUS®AAV-Helper

pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in suspension. The use of pPLUS® AAV-Helper leads to enhanced AAV titers and improved AAV particles quality.

This plasmid is the outcome of an extensive R&D program based on our proprietary DNA assembly technology (the eZyvec® technology) aiming at exploring the synergies of multiple genetic features to improve the infectivity (measured in TU/mL) and quality (full/empty ratio) of the viral particles obtained through triple transfection of HEK293 cells in suspension with FectoVIR®-AAV. They are readily available as an off-the-shelf product.
Key Benefits:
Optimized for AAV Production with FectoVIR®-AAV: Increased AAV titers and AAV particles quality when used together with FectoVIR®-AAV
Versatile: Suitable for use with various cell lines and production of several AAV serotypes.
Compatible: With PEIpro® and other transfection reagents, suitable for use with various cell lines and production of several AAV serotypes.
Adapted for your Projects from Research to Large Scale Manufacturing: Three packsize of Research Grade available, HQ and GMP Grade. GMP Grade coming soon (2025).

Specifications
General Specifications:
Amount of Reagent: pPLUS® AAV-Helper 100 mg