Sartorius, 110.5114-1.3, CIMac DEAE 0.1 mL Analytical Column (1.3 µm)

CIMac Adeno Analytical Columns are intended for fast and reproducible analysis and quantification of Adenovirus samples at different stages of downstream and upstream.

CIMac Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short column length allow the operation at high volumetric flow rates, enabling them to receive the information about the product quantity and purity in just a few minutes. Hence, the CIMac Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps.
The CIMac DEAE-0.1 Analytical Columns are weak anion-exchange monolithic columns, that bind biomolecules that are predominantly negatively charged over the pH working range of 3-9.
DEAE (diethylamine) features:
Weak anion exchanger
Working range, pH 2–9
Binds molecules with predominantly negative charge, repels molecules a predominantly positive charge
Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for plasmid DNA
Sanitizable with 1 M NaOH

Specifications
Applications:
Application: Analytical Columns

Connections (Physical):
Connector(s): 10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions:
Channel Size: 1.3 µm
Diameter: 5.2 mm
Length: 4.95 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min

Materials of Construction:
Materials of Construction: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 15 MPa | 150 bar | 2175 psi
Recommended Operating Temperature: 4 °C (39 °F) to 40 °C (104 °F)
pH Stability: Working range 2–10, cleaning in place 1–14

Physicochemical Information:
Bed Volume: 0.1 mL
Ligand: DEAE (weak anion exchanger, diethylamino)
Typical Dynamic Binding Capacity 10% per mL: 25–31 mg BSA/mL wet support, BSA, 0.5 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flow rate 1 mL/min

Product Information:
Brand: CIMac™