Sartorius, 4440, Incucyte® Caspase-3/7 Dye for Apoptosis

One vial (20 µL) of mix-and-read reagent in DMSO sufficient for 100-200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.
Top Features of Incucyte® Caspase-3/7 Dye for Apoptosis
Reduce loss of precious or compromised cells under-going caspase-3/7 mediated apoptosis with mix-and-read reagents

Incucyte® Caspase-3/7 Dyes for Apoptosis are formulated to detect apoptosis (cell death) in live cells. The dyes are ideally suited to the mix-and-read, real-time quantification of cells undergoing caspase-3/7 mediated apoptosis. Addition of the Incucyte® Caspase-3/7 Green, Red or Metabolism Dyes to healthy cells is non-perturbing to cell growth and morphology. When used with Incucyte® Live-Cell Analysis Systems and software, these reagents allow for reliable, accurate evaluation of cell death. Incucyte® Caspase-3/7 Dyes for Apoptosis couple the activated caspase-3/7 recognition motif (DEVD) to a DNA intercalating dye. When added to tissue culture medium, the inert, non-fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7, resulting in the release of the DNA dye and fluorescent staining of the nuclear DNA. With Incucyte® integrated analysis software, fluorescent objects can be quantified and background fluorescence minimized. These reagents have been validated for use with the Incucyte® Live-Cell Analysis System and enable real-time evaluation of cell apoptosis induced by pharmacological agents and/or genetic and environmental factors.
Presentation, Storage and Stability
The dyes are supplied as single-vial solutions in dimethyl sulfoxide (DMSO). Each vial provides a sufficient quantity for performing 100-200 tests (one test is one well of a 96-well microtiter plate). Upon receipt, the Incucyte® Caspase 3/7 Dyes should be stored at 4°C where they are stable for six months.
Recommended Use
We recommend diluting Incucyte® Caspase-3/7 Dyes in complete culture media and adding directly to cells in culture, as per the guidelines below:
Incucyte® Caspase-3/7 Green: 1:1000 in culture media, for a final assay concentration of 5 μM
Incucyte® Caspase-3/7 Red: Optimize final assay concentration by diluting the reagent 1:200 in complete medium, then make a series of 2-fold dilutions for final assay concentrations of 2.5, 1.25 and 0.5 µM
Incucyte® Caspase-3/7 for Metabolism: 1:1000 in culture media, for a final assay concentration of 1 μM
Additional protocol details can be found in the Product Guide under the Documents tab.

Specifications
Applications:
Application: Apoptosis
Assay Type: Cell Health; Viability
Research Area: Cell Health

Features:
Device/Instrument Compatibility: S3; SX1; SX5
Optical Module: Green/Red; Green/Orange/NIR

General Specifications:
Color: Green

Product Information:
Brand: Incucyte®
Pack Size: 1